AbstractObjectivesThe purpose of this study was to investigate the anti-inflammatory effect of Cornus Officinalis fruit extract(CE) and Cornus Officinalis Fruit Cheonghyeol Plus(CCP) in Human Umbilical Vein Endothelial Cell.
MethodsWe measured cell viability of CE, CCP and treated HUVEC with TNF-α. We measured the mRNA expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, the protein expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, and the protein phosphorylation level of ERK, JNK, p38 and the biomarker expression levels of MCP-1, ICAM-1, VCAM-1.
Results1.CE incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100μg/ml compared to the control group. CE decresed the mRNA, protein and biomarker expression levels of MCP-1,ICAM-1,VCAM-1 at concentrations of 100μg/ml compared to the control group. CE decresed the protein phosphorylation level of p38 at concentrations of 100μg/ml compared to the control group. 2. CCP incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100μg/ml or more compared to the control group. CCP decresed the mRNA, protein and biomarker expression levels of MCP-1, ICAM-1, VCAM-1 at concentrations of 100μg/ml or more compared to the control group. CCP decresed the protein phosphorylation level of ERK at concentrations of 100μg/ml or more, p38 at concentrations of 200μg/ml or more, and JNK at concentrations of 400μg/ml compared to the control group.
참고문헌1. Oh B.H.2003; New Concepts in the pathogenesis and Progression of Atherosclerosis. Medical postgraduates. 31:4. 179–183.
2. Statistics Korea. 2021. 2020 Death statistics : Korea. Korea development institute;Available from: URL: http://kostat.go.kr/portal/korea/kor_nw/1/6/2/index.board?bmode=read&bSeq=&aSeq=403046&pageNo=1&rowNum=10&navCount=10&currPg=&searchInfo=&sTarget=title&sTxt=
3. Jeong H.S.2019. Risks and Management of Dyslipidemia. Public health weekly report. 2019. 12:37. 1416–1211. Available from: URL: https://www.kdca.go.kr/board/board.es?mid=a20602010000&bid=0034&list_no=364839&act=view
4. Bae J.H., Park J.S., Hond G.R., Shin D.G., Kim Y.J., Shim B.S.2008; Correlation between inflammatory markers and the progression of atherosclerosis in patients with coronary artery disease. The Korean Journal of Medicine. 74:1. 51–58.
5. Stenvinkel P.2003; Interactions betwen inflammation, oxi dative stres, and endothelial dysfunction in end-stage re nal disease. J Ren Nutr. 13:2. 144–148.
https://doi.org/10.1053/jren.2003.50018
6. Wolf D., Ley K.2019; Immunity and Inflammation in Atherosclerosis. Circ Res. 124:2. 315–327.
https://doi.org/10.1161/CIRCRESAHA.118.313591
7. Glass C.K., Witztum J.L.2001; Atherosclerosis.The road ahead. Cell. 104:4. 503–516.
https://doi.org/10.1016/s0092-8674(01)00238-0
8. Seo D.H., Joo I.H., Kim D.H.2018; Effect of ChungHuyl-Plus on inflammatory factors in Human Umbilical Vein Endothelial Cells (HUVECs). J Haehwa Medicine. 27:2. 11–20.
9. Oh J.M., Cho H.K., Yoo H.R., Kim Y.S., Seol I.C.2016; A Case Report of the Beneficial Effects of Chunghyul-Plus in Dyslipidemia Patients. The Journal of the Society of Stroke on Korean Medicine. 17:1. 55–66.
10. Park E.B., Kim H.S., Shin S.Y., Ji I.A., Kim J.H., Kim S.G., et al2021; Antioxidative Activity of Cornus officianalis Extracts Obtained by Four Different Extraction Techniques. Journal of Life Science. 22:11. 1507–1514.
http://dx.doi.org/10.5352/JLS.2012.22.11.1507
11. Kim Y.J., Son D.Y.2016; Antioxidant activity and suppression of pro-inflammatory mediator of Corni fructus extracts in activated. Korean J Food Preserv. 23:6. 876–882.
https://doi.org/10.11002/kjfp.2016.23.6.876
12. Shin J.H., Cha G.Y., Kim H.J., Hwang J.H., Han K.H., Seo H.J., et al2009; Exmination of Anti-Obesity Effect of RegionalSpecial Natural Products of Anthrisci radix, Psoraleaesemen, Siegesbeckiae herba and Corni fructus. KSBB Journal. 24:6. 549–555.
13. Yamabe N., Noh J.S., Park C.H., Kang K.S., Shibahara N., Tanaka T., et al2010; Evaluation of loganin, iridoid glycoside from Corni Fructus, on hepatic and renal glucolipotoxicity and inflammation in type 2 diabetic db/db mice. European Journal of Pharmacology. 648:13. 179–187.
https://doi.org/10.1016/j.ejphar.2010.08.044. Epub 2010 Sep 15
14. Jiang Z.Q., Li Y., Jiang L.H., Gu H., Wang M.Y.2013; Hepatoprotective effects of extracts from processed corni fructus against D-galactose-induced liver injury in mice. Zhong Yao Cai. 36:1. 85–89.
15. Ross R.1993; The pathogenesis of atherosclerosis: A perspective for the 1990s. Nature. 362:6423. 801–809.
https://doi.org/10.1038/362801a0
16. Committee of Clinical Practice Guideline of the Korean Society of Lipid and Atherosclerosis (KSoLA). 2018. Korean Guidelines for the Management of Dyslipidemia. 4th ed. Seoul: p. 31–44.
17. Li J.J., Fang C.H.2004; Atheroscleritis is a more rational term for the pathological entity currently known as atherosclerosis. Med Hypotheses. 63:1. 100–102.
https://doi.org/10.1016/j.mehy.2004.01.029
18. Bergheanu S.C., Bodde M.C., Jukema J.W.2017; Pathophysiology and treatment of atherosclerosis. Neth Heart J. 25:4. 231–242.
https://doi.org/10.1007/s12471-017-0959-2
19. Yang Y.K.1991. Hwangjenegyung-yuckhe. Seoul: Iljoong-sa.
20. Yang J.H., Yoo H.R., Kim Y.S., Seol I.C.2021; The Effect of Lonicera Japonica Thunberg on Inflammatory Factor Expression Associated with Atherosclerosis. Korean J. Orient. Int. Med. 42:1. 25–39.
https://doi.org/10.22246/jikm.2021.42.1.25
21. Do H.J., Kim K.H., Oh T.W.2020; Anti-hyperlipidemic Effects of Scutellariae Radix, Aucklandiae Radix and Bupleuri Radix (SAB) extract in FL83B cells. Kor. J. Herbol. 35:5. 23–31.
https://doi.org/10.6116/kjh.2020.35.5.23
22. Han B.H., Yoon J.J., Kim H.Y., Ahn Y.M., Hong M.H., Son C.O., et al2018; Inhibitory Effects of Ojeoksan on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells. Kor. J. Herbol. 33:4. 59–67.
https://doi.org/10.6116/kjh.2018.33.4.59
23. Lee K.W., Cho H.K., Yoo H.R., Seol I.C.2018; The Effects of an Extract of Fermented Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix on Anti-inflammation Associated with Dyslipidemia and Anti-oxidation in RAW264.7 and HUVEC Cells. J. Int. Korean Med. 39:4. 480–494.
https://doi.org/10.22246/jikm.2018.39.4.480
24. Choi K.E., Seol I.C., Kim Y.S., Jo H.K., Yoo H.R.2016; Hypolipidemic and Anti-oxidant Effects of Chunghyl Plus in Type II Diabetic Mice Model. J Physiol & Pathol Korean Med. 30:3. 164–176.
https://doi.org/10.15188/kjopp.2016.06.30.3.164
25. Lee J.H., Jang H.J., Kim K.S., Yang H.J., Lee J.Y., Na Y.C., et al2014; Effects of β-sitosterol derived from Artemisia capillaris on the activated human hepatic stellate cells and dimethylnitrosamine-induced mouse liver fibrosis. BMC Complement Altern Med. 14:363. 1–10.
https://doi.org/10.1186/1472-6882-14-363
26. Oh D.Y., Kang D.S., Lee Y.G., Kim H.S.2019; Effects of Turmeric (Curcuma longa L.) on Blood Glucose and Lipid Metabolism Functional Improvement in STZ-induced Diabetic rats. J Environmental Science International. 28:5. 485–494.
https://doi.org/10.5322/JESI.2019.28.5.485
27. Oh D.Y., Kim H.S.2019; Evaluation of Oxidation Inhibition and Nitrogen Oxide Scavenging Activity from Curcuma longa L. Extracts. J Kor. applied science and technology. 36:1. 13–22.
https://doi.org/10.12925/jkocs.2019.36.1.13
28. Kwon S.H., Kim J.B.2010; Effects of Crataegii Fructus on the Diet-induced Hyperlipidemia in Rats. Korean J. Oriental Physiology & Pathology. 24:1. 67–73.
29. Lee S.E., Cho S.I.2015; Anti-inflammatory effects of Salviae Miltiorrhizae Radix extract on RAW264.7 cell. via anti-oxidative activities. Kor. J. Herbol. 30:4. 89–94.
http://dx.doi.org/10.6116/kjh.2015.30.4.89
30. Zhang J., Liang R., Wang L., Yang B.2019; Effects and mechanisms of Danshen-Shanzha herb-pair for atherosclerosis treatment using network pharmacology and experimental pharmacology. J Ethnopharmacol, 2019. 229:1. 104–114.
https://doi.org/10.1016/j.jep.2018.10.004
31. Packard R.R.S., Libby P.2008; Inflammation in atherosclerosis: from vascular biology to biomarker discovery and risk prediction. Clin Chem. 54:1. 24–38.
https://doi.org/10.1373/clinchem.2007.097360
32. Onat D., Brillon D., Colombo P.C., Colombo P.C., Schmidt A.M.2011; Human Vascular Endothelial Cells: A Model System for Studying Vascular Inflammation in Diabetes and Atherosclerosis. Curr Diab Rep. 11:3. 193–202.
https://doi.org/10.1007/s11892-011-0182-2
33. Bu D., Tarrio M., Grabie N., Zhang Y., Yamazaki H., Stavrakis G., et al2010; Statin-induced Kruppel-like factor 2 expression in human and mouse T cells reduces inflammatory and pathogenic responses. J Clin Invest. 120:6. 1961–1970.
https://doi.org/10.1172/JCI41384. Epub 2010 May 3
34. Boon R.A., Horrevoets A.J.2009; Key transcriptional regulators of the vasoprotective effects of shear stress. Hamostaseologie. 29:1. 39–43.
35. Yim C.Y.2010; Nitric oxide and cancer. Korean J Med. 78:4. 430–436.
36. Shin J.J., Lee S.Y., Lee S.H., Lee S.H., Suh J.K., Cho J.Y., et al1996; The Role and Localization of Nitric Oxide Synthase in Neurogenic Inflammation of the Rat Airways. Tuberculosis and Respiratory disease. 43:3. 420–432.
https://doi.org/10.4046/trd.1996.43.3.420
37. Ramji D.P., Davies T.S.2015; Cytokines in atherosclerosis: Key players in all stages of disease and promising therapeutic targets. Cytokine Growth Factor Rev. 26:6. 673–685.
https://doi.org/10.1016/j.cytogfr.2015.04.003. Epub 2015 May 12
|
|