The Effect of Gamisoyo-san Intravenous Pharmacopuncture on Restoration of Liver Function after Partial Hepatectomy in SD Rat

Chan Lee; Jung-Hun Yun; Yun-kyoung Yim; Lee, Chan; Yun, Jung-Hun; Yim, Yun-kyoung

doi:10.13048/jkm.15003

JKM > Volume 36(1); 2015 > Article

Lee, Yun, and Yim: The Effect of Gamisoyo-san Intravenous Pharmacopuncture on Restoration of Liver Function after Partial Hepatectomy in SD Rat

Original Article

The Journal of Korean Oriental Medicine 2015; 36(1): 22-32.

DOI: https://doi.org/10.13048/jkm.15003

The Effect of Gamisoyo-san Intravenous Pharmacopuncture on Restoration of Liver Function after Partial Hepatectomy in SD Rat

Chan Lee, Jung-Hun Yun, Yun-kyoung Yim

Dept. of Meridian and Acupoint, College of Oriental Medicine, Daejeon University

Correspondence to : Yun-Kyoung Yim, College of Korean Medicine. Daejeon University., #12407, 96-3, Yongun-dong, Dong-gu, Daejeon, 300-716 South Korea, Tel : +82-42-280-2610, Fax : +82-42-280-2641, E-mail : docwindy@dju.kr

Received January 21, 2015 Revised March 13, 2015 Accepted March 17, 2015

Abstract

Objectives:

This study researched the effect of Gamisoyo-san intravenous pharmacopuncture on restoration of liver function after partial hepatectomy in SD rat.

Methods:

Twelve rats were randomly divided into three groups: control group (n=4) underwent partial hepatectomy, saline group (n=4) was injected 1ml saline after partial hepatectomy, Gamisoyo-san group (n=4) was injected with 10mg/kg Gamisoyo-san after partial hepatectomy. Liver function and liver regeneration ratio were measured on the seventh day after partial hepatectomy.

Results:

The results show that the AST, AST and ALT level in Gamisoyo-san group was significantly lower than those of control group and saline group (P<0.05)

Conclusion:

In partial hepatectomy model, Gamisoyo-san intravenous pharmacopuncture seems to significantly promote the restoration of liver function.

Keywords: Gamisoyo-san, intravenous pharmacopuncture, partial hepatectomy, liver function

Fig. 1.

Serum AST in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.

Each value and vertical bar represent the mean±S.D. ^*P<0.05, vs control group by Mann-Whitney Test, ^†P<0.05, vs saline group by Mann-Whitney Test

Fig. 2.

Serum ALT in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.

Each value and vertical bar represent the mean±S.D. ^*P<0.05, vs control group by Mann-Whitney Test, ^†P<0.05, vs saline group by Mann-Whitney Test

Fig. 3.

Serum ALP in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.

Each value and vertical bar represent the mean±S.D. ^*P<0.05, vs control group by Mann-Whitney Test, ^†P<0.05, vs saline group by Mann-Whitney Test

Fig. 4.

Liver regeneration ratio in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.

Each value and vertical bar represent the mean±S.D.

Table 1.

Composition of Gamisoyo-san and Amount of Herbs Used

Name of Herbs	Scientific Name	Original amount (g)	Amount used (g)
목단피	Paeonia suffruticosa	6g	2.25g
백출	Atractylodes japonica	6g	2.25g
적작약	Paeonia lactiflora	4g	1.5g
당귀	Angelica gigas	4g	1.5g
도인	Prunus persica	4g	1.5g
패모	Fritillaria ussuriensis	4g	1.5g
치자	Gardenia jasminoides	3.2g	1.2g
황금	Scutellaria baicalensis	3.2g	1.2g
길경	Platycodon grandiflorum	2.8g	1.05g
청피	Citrus unshiu	2g	0.75g
감초	Glycyrrhiza uralensis	1.2g	0.45g

Sum		40.4g	15.15g

Table 2.

Serum AST, ALT, ALP 7 Days after Partial Hepatectomy Values Represent Mean±S.D. (n=4)

	Control group	Saline group	Gamisoyo-san group
AST	172.35±88.16	227.52±139.02	81.32±9.23
ALT	73.2±32.65	48.65±6.64	31.75±10.35
ALP	1228.4±172.83	1102.02±251.43	741.75±74.66

Table 3.

Liver Regeneration Ratio in Control Group and Saline Group, Gamisoyo–San Group 7 Days After Partial Hepatectomy

Group	resected liver (g)	regenerated liver (g)	liver regeneration ratio (%)
control group	3.84±0.41	11.46±0.82	82
saline group	3.82±0.51	9.99±2.10	71.5
Gamisoyo-san Group	3.89±0.65	12.27±1.06	87.8

Values represent mean ± S.D. (n=4)

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