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JKM > Volume 36(1); 2015 > Article
Lee, Yun, and Yim: The Effect of Gamisoyo-san Intravenous Pharmacopuncture on Restoration of Liver Function after Partial Hepatectomy in SD Rat

Abstract

Objectives:

This study researched the effect of Gamisoyo-san intravenous pharmacopuncture on restoration of liver function after partial hepatectomy in SD rat.

Methods:

Twelve rats were randomly divided into three groups: control group (n=4) underwent partial hepatectomy, saline group (n=4) was injected 1ml saline after partial hepatectomy, Gamisoyo-san group (n=4) was injected with 10mg/kg Gamisoyo-san after partial hepatectomy. Liver function and liver regeneration ratio were measured on the seventh day after partial hepatectomy.

Results:

The results show that the AST, AST and ALT level in Gamisoyo-san group was significantly lower than those of control group and saline group (P<0.05)

Conclusion:

In partial hepatectomy model, Gamisoyo-san intravenous pharmacopuncture seems to significantly promote the restoration of liver function.

Fig. 1.
Serum AST in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D. *P<0.05, vs control group by Mann-Whitney Test, P<0.05, vs saline group by Mann-Whitney Test
jkm-36-1-22f1.tif
Fig. 2.
Serum ALT in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D. *P<0.05, vs control group by Mann-Whitney Test, P<0.05, vs saline group by Mann-Whitney Test
jkm-36-1-22f2.tif
Fig. 3.
Serum ALP in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D. *P<0.05, vs control group by Mann-Whitney Test, P<0.05, vs saline group by Mann-Whitney Test
jkm-36-1-22f3.tif
Fig. 4.
Liver regeneration ratio in control group, saline group and Gamisoyo-san group 7 days after partial hepatectomy.
Each value and vertical bar represent the mean±S.D.
jkm-36-1-22f4.tif
Table 1.
Composition of Gamisoyo-san and Amount of Herbs Used
Name of Herbs Scientific Name Original amount (g) Amount used (g)
목단피 Paeonia suffruticosa 6g 2.25g
백출 Atractylodes japonica 6g 2.25g
적작약 Paeonia lactiflora 4g 1.5g
당귀 Angelica gigas 4g 1.5g
도인 Prunus persica 4g 1.5g
패모 Fritillaria ussuriensis 4g 1.5g
치자 Gardenia jasminoides 3.2g 1.2g
황금 Scutellaria baicalensis 3.2g 1.2g
길경 Platycodon grandiflorum 2.8g 1.05g
청피 Citrus unshiu 2g 0.75g
감초 Glycyrrhiza uralensis 1.2g 0.45g

Sum 40.4g 15.15g
Table 2.
Serum AST, ALT, ALP 7 Days after Partial Hepatectomy Values Represent Mean±S.D. (n=4)
Control group Saline group Gamisoyo-san group
AST 172.35±88.16 227.52±139.02 81.32±9.23
ALT 73.2±32.65 48.65±6.64 31.75±10.35
ALP 1228.4±172.83 1102.02±251.43 741.75±74.66
Table 3.
Liver Regeneration Ratio in Control Group and Saline Group, Gamisoyo–San Group 7 Days After Partial Hepatectomy
Group resected liver (g) regenerated liver (g) liver regeneration ratio (%)
control group 3.84±0.41 11.46±0.82 82
saline group 3.82±0.51 9.99±2.10 71.5
Gamisoyo-san Group 3.89±0.65 12.27±1.06 87.8

Values represent mean ± S.D. (n=4)

참고문헌

1.. Arthur C, John E. Textbook of Medical Physiology. 11th ed.Philadelphia: Elsevier Saunders;2005. p. 859–864.


2.. Credland N. Interpreting Blood Results - Liver Function Tests. Dermatological Nursing. 2014; 13:2. 42–45.


3.. Ministry of Health & Welfare. The Fifth Korea National Health and Nutrition Examination Survey (KNHANES V-2). Seoul: Korea Centers for Disease Control and Prevention;2011. p. 548–693.


4.. Vinay K, Abul K, John C. Robbins Basic Pathology. 9th ed.Philadelphia: Elsevier Science Health Science div;2013. p. 637


5.. Llovet JM, Burroughs A, Bruix J. Hepatocellular Carcinoma. Lancet. 2003; 362:9399. 1907–1917.
crossref

6.. Jung KW, Won YJ, Kong HJ, Oh CM, Lee DH, Lee JS. Cancer Statistics in Korea: Incidence, Mortality, Survival and Prevalence in 2011. Cancer Research and Treatment. 2014; 46:2. 109–123.
crossref

7.. Bae SH. Up-to-date Information for Hepatocellular Carcinoma Treatment. J Korean Med Assoc. 2008; 51:5. 457–474.
crossref

8.. Yoo HC. Surgical Strategies in hepatocarcinoma treatment. Korean J Gastroenterol. 2007; 49:4. 226–235.


9.. George K. Liver Regeneration after Partial Hepatectomy. Am J Pathol. 2010; 176:1. 2–13.
crossref

10.. Lee S, Lee M. Present status of Chinese medicine intervention for treatment of the liver cancer: application base and clinical research. Beijing J Tradit Chin Med. 2009; 28:2. 145–147.


11.. Bae SH, Park SE, Kang CW, Hong SH. Safety of Korean Herbal Medicine Used with Western Medicine on Liver Function: Prospective Observational Study. Korean J Orient Int Med. 2013; 34:2. 192–203.


12.. Yun YJ, Shin BC, Lee MS, Cho SI, Shin WJ, Park HJ, et al. Korean Herbal Medicine on Liver Function: A Systematic Review in Korean Literature. Korean J Orient Int Med. 2009; 30:1. 153–172.


13.. Zhao XX. Studiess of Chinese Herbal Injections. Guangzhou: Guangdong Science & Technology Press;2000. p. 3–4.


14.. Zhou JM, Zhang JQ. Pharmacology and Application of Chinese Patent Drug. Shanghai: Fudan University Press;2003. p. 20


15.. Bang SH, Kwon KR, Yoo HS. Two Cases of Non-Small Cell Lung Cancer Treated with Intravenous Cultivated Wild Ginseng Pharmacopuncture. J Pharmacopunct. 2008; 11:2. 13–19.
crossref

16.. Lee DN, Yang WM, Lee TK, Lim EM. A Study of Sayuk-san and Soyo-san for Clinical Using. J Korean Med Obstet Gynecol. 2000; 13:1. 484–499.


17.. Lee SH, Kim KS, Yun JM, Ko HN, Kim LH, Rhee YJ, et al. Case Report on Three Cases of Postoperative Pain after Thyroidectomy Treated with Gamisoyo-san. Korean J Orient Physiol Pathol. 2012; 26:5. 793–796.


18.. Kim HJ. Effect of “Kami-Soyo-San” Ex on the Hepatic Damages induced by CCl4 in rats [dissertation]. Gwangju: Chosun Univ;1986.


19.. Gaub J, Iverson J. Rat liver regeneration after 90% partial hepatectomy. Hepatology. 1984; 4:902–904.
crossref

20.. Ramzi S, Vinay K, Nelson F, Stanley L, Abul K. Robbins and Cotran pathologic basis of disease. 7th ed.Philadelphia: Elsevier Saunders;2005. p. 878


21.. Lee WB, Ryu CS, Kim KY. Evidences of the Intravascular Erythropoiesis in Human Fetal Liver. Korean J Anat. 1995; 28:4. 351–364.


22.. Gerard J, Bryan D. Principles of anatomy and physiology. 12th ed.Hoboken: Wiley;2009. p. 945–949.


23.. Park BG, Yoon SG, Lee HS, Chang JG, Maeng JH, Lee GH, et al. Epidemiological comparison of viral hepatitis-hepatocellular carcinoma (HCC) and non viral hepatitis-hepatocellular carcinoma (HCC). Korean J Epidemiol. 2003; 25:1. 32–38.


24.. Centers for Disease Control and Prevention. Surveillance for Viral Hepatitis – United States 2011. Atlanta: Centers for Disease Control and Prevention;2011.


25.. Kim HJ, Hwang YJ, Kim JY, Kwon HJ, Kim GS, Chun JM, et al. Comparison of Surgical Outcomes and Long-term Survival between Limited and Major Hepatic Resection for Hepatocellular Carcinoma Less than 5 cm. J Korean Surg Soc. 2010; 78:29–34.
crossref

26.. Lin TY, Chen CC. Metabolic function and regeneration of cirrhotic and non-cirrhotic livers after hepatic lobectomy in man. Ann Surg. 1965; 162:6. 959–972.
crossref

27.. Takenaka K, Kanematsu T, Fukuzawa K, Sugimachi K. Can hepatic failure after surgery for hepatocellular carcinoma in cirrhotic patients be prevented? World J Surg. 1990; 14:1. 123–127.
crossref

28.. Kim GS, Lee SM, Hong SH, Joo HJ. Perioperative Risk Factors of the Hepatic Resection. Ann Surg Treat Res. 1998; 55:4. 556–563.


29.. Baek DK. The Clinical Study of Two Severe Menopausal Disorder Patients Treated by Gamisoyo-san. Herbal Formula Science. 2012; 20:2. 199–211.


30.. Park SW. Antidepressant Action of Kamishoyosan [dissertation]. Busan: Inje Univ;2004.


31.. Yang SK, Yong JS. Perception of Artificial Hydration for Terminally Ill Cancer Patients: Patients, Families and General Public. Korean J Hosp Palliat Care. 2009; 12:4. 220–227.


32.. Yang GM. Pharmacy of Chinese Medicine. Beijing: People’s Medical Publishing House;2010. p. 156–184.


33.. Kim K, Choi YS, Joo JC, Moon G. A Case Report for Lung Cancer Patient Showing Remission Treated with Cultivated Wild Ginseng Pharmacopuncture. J Pharmacopunct. 2011; 14:4. 33–37.
crossref

34.. Han DH. The significance of the actual half-life of serum alpha-fetoprotein as a predictor for recurrence after curative hepatectomy in patients with hepatocellular carcinoma [dissertation]. Seoul: Yonsei Univ;2011.


35.. Johnson DC. Exotic Animal Companion Medicine Handbook for Veterinarians. West Palm Beach: Zoological Education Network;1996. p. 9–98.


36.. Jeong SO. penta-O-galloyl-β-D-glucose upregulates heme oxygenase-1 expression by stimulating Nrf2 nuclear translocation in an extracellular signal-regulated kinase-dependent manner in HepG2 cells [dissertation]. Jeonju: Chonbuk Univ;2006.


37.. Cheng YY, Chan YS, Choang KF, Chang HM. Effect of geniposide on acute jaundice in rats caused by ANIT poisoning. Acta Pharmacol Sin. 1986; 7:1. 69–72.


38.. Matsumi Y, Kenzo C. Genipin exhibits neurotrophic effects through a common signaling pathway in nitric oxide synthase-expressing cells. Eur J Pharmacol. 2008; 581:3. 255–261.
crossref

39.. Na JX, Park EJ, Sohn DH, Ko GI. Hepatoprotective and antifibrotic effects of extracts from Persicae Semen. Herbal Formula Science. 2004; 12:2. 109–117.


40.. Lim JG, Moon JJ. Experimental Studies on the Effect of Dangkwisoo-San on the CCl4 −Induce dliver Damage in Albino-Rats. KHUniversity Orient Med J. 1982; 5:191–208.


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