Effects of Schisandrae Fructus on menopause symptoms in ovariectomized mice

Dasom Shin; Seong Bin Hong; Jeong Ho Geum; Jin Yeul Ma; Hwan-Suck Chung; Shin, Dasom; Hong, Seong Bin; Geum, Jeong Ho; Ma, Jin Yeul; Chung, Hwan-Suck

doi:10.13048/jkm.16033

JKM > Volume 37(3); 2016 > Article

Shin, Hong, Geum, Ma, and Chung: Effects of Schisandrae Fructus on menopause symptoms in ovariectomized mice

Original Article

The Journal of Korean Medicine 2016; 37(3): 39-46.

Published online: September 30, 2016

DOI: https://doi.org/10.13048/jkm.16033

Effects of Schisandrae Fructus on menopause symptoms in ovariectomized mice

Dasom Shin¹, Seong Bin Hong², Jeong Ho Geum², Jin Yeul Ma³, Hwan-Suck Chung³

¹Department of Physiology, College of Korean Medicine, Kyung Hee University

²BIOMIX Inc.

³Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)

Correspondence to: 정환석 (Hwan-Suck Chung), 대구광역시 첨단로 70 한국한의학연구원 한의기술응용센터, Tel: +82-53-940-3875, Fax: +8253-940-3849, E-mail: hschung@kiom.re.kr

Received August 30, 2016 Revised September 20, 2016 Accepted September 20, 2016

Abstract

Objectives

The present study aimed at investigating the effects of Schisandrae Fructus (SF) on menopause symptoms in ovariectomized mice.

Methods

6 weeks old female mice were ovariectomized (OVX), and the OVX mice were fed with a three doses of SF (25, 100, and 400 mg/kg, respectively) or 17β-estradiol (E2, 0.5 mg/kg) 3 times a week for 6 weeks. Body weight was measured at every week and uterus weight was also measured at the end of experiment. Lipids and osteocalcin levels in serum were analyzed.

Results

The uterine weight was not changed in SF treated group, while E2 intake increased the reduced uterine weight by OVX. Although Body weight was increased since two weeks after ovariectomy compared with sham operated group, there was no change in SF or E2 treated group compared with OVX. But LDL was significantly reduced in SF (400 mg/kg) or E2 fed mice and SF or E2 treatment decreased enhanced osteocalcin level by OVX.

Conclusion

These results indicated that SF would be effective in the improvement of LDL level and osteoporosis on menopause.

Keywords: Menopause, Schisandrae fructus, Ovariectomized mice, Osteoporosis, Osteocalcin

Fig. 1

HPLC analysis of SCB. The structure of Gomisin N(A), Calibration curve of Gomisin N (B), Chromatogram of SCB (C) and Gomisin N (D) using HPLC systems.

Fig. 2

Effects of SCB on uterus weight in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (***p<0.001 vs Sham and, ***p<0.001 vs OVX+Vehicle).

Fig. 3

Effects of SCB on body weight in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (***p<0.001, **p<0.01, *p<0.05 vs Sham).

Fig. 4

Effects of SCB on serum lipids in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (**p<0.01, *p<0.05 vs Sham and, ###p<0.001, #p<0.05 vs OVX+Vehicle).

Fig. 5

Effects of SCB on osteocalcin in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (**p<0.01 vs Sham and, ***p<0.001, **p<0.01 vs OVX+Vehicle).

Table 1

Experimental Design of Animal

Group	Operation	Treatment
Sham	sham-operated
OVX-Vehicle	ovariectomized
OVX-E2	ovariectomized	Estradiol 0.5 mg/kg bw/day
OVX-SCB25	ovariectomized	Schisandra chinensis Baillon 25 mg/kg bw/day
OVX-SCB100	ovariectomized	Schisandra chinensis Baillon 100 mg/kg bw/day
OVX-SCB400	ovariectomized	Schisandra chinensis Baillon 400 mg/kg bw/day

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