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JKM > Volume 37(3); 2016 > Article
Shin, Hong, Geum, Ma, and Chung: Effects of Schisandrae Fructus on menopause symptoms in ovariectomized mice



The present study aimed at investigating the effects of Schisandrae Fructus (SF) on menopause symptoms in ovariectomized mice.


6 weeks old female mice were ovariectomized (OVX), and the OVX mice were fed with a three doses of SF (25, 100, and 400 mg/kg, respectively) or 17β-estradiol (E2, 0.5 mg/kg) 3 times a week for 6 weeks. Body weight was measured at every week and uterus weight was also measured at the end of experiment. Lipids and osteocalcin levels in serum were analyzed.


The uterine weight was not changed in SF treated group, while E2 intake increased the reduced uterine weight by OVX. Although Body weight was increased since two weeks after ovariectomy compared with sham operated group, there was no change in SF or E2 treated group compared with OVX. But LDL was significantly reduced in SF (400 mg/kg) or E2 fed mice and SF or E2 treatment decreased enhanced osteocalcin level by OVX.


These results indicated that SF would be effective in the improvement of LDL level and osteoporosis on menopause.

Fig. 1
HPLC analysis of SCB. The structure of Gomisin N(A), Calibration curve of Gomisin N (B), Chromatogram of SCB (C) and Gomisin N (D) using HPLC systems.
Fig. 2
Effects of SCB on uterus weight in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (***p<0.001 vs Sham and, ***p<0.001 vs OVX+Vehicle).
Fig. 3
Effects of SCB on body weight in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (***p<0.001, **p<0.01, *p<0.05 vs Sham).
Fig. 4
Effects of SCB on serum lipids in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (**p<0.01, *p<0.05 vs Sham and, ###p<0.001, #p<0.05 vs OVX+Vehicle).
Fig. 5
Effects of SCB on osteocalcin in ovariectomized mice (OVX). Data are shown as mean ± S.E.M. Statistical analyses were conducted by one-way ANOVA (**p<0.01 vs Sham and, ***p<0.001, **p<0.01 vs OVX+Vehicle).
Table 1
Experimental Design of Animal
Group Operation Treatment
Sham sham-operated
OVX-Vehicle ovariectomized
OVX-E2 ovariectomized Estradiol 0.5 mg/kg bw/day
OVX-SCB25 ovariectomized Schisandra chinensis Baillon 25 mg/kg bw/day
OVX-SCB100 ovariectomized Schisandra chinensis Baillon 100 mg/kg bw/day
OVX-SCB400 ovariectomized Schisandra chinensis Baillon 400 mg/kg bw/day


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