The Effect of  Nakai Extract and  KJS-2 on Atopic Dermatitis induced by DNCB in mice

Deok-Hyun Ryu; Sa-Rang Oh; Tae-Sung Jung; Deok-Seon Ryu; Ryu, Deok-Hyun; Oh, Sa-Rang; Jung, Tae-Sung; Ryu, Deok-Seon

doi:10.13048/jkm.17023

JKM > Volume 38(3); 2017 > Article

Ryu, Oh, Jung, and Ryu: The Effect of Angelica gigas Nakai Extract and Bacillus Polyfermenticus KJS-2 on Atopic Dermatitis induced by DNCB in mice

Original Article

The Journal of Korean Medicine 2017; 38(3): 30-42.

Published online: September 30, 2017

DOI: https://doi.org/10.13048/jkm.17023

The Effect of Angelica gigas Nakai Extract and Bacillus Polyfermenticus KJS-2 on Atopic Dermatitis induced by DNCB in mice

Deok-Hyun Ryu¹, Sa-Rang Oh², Tae-Sung Jung², Deok-Seon Ryu³^{, *}

¹Dept. of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Dae-jeon University

²Wellbeingtainment Co., Ltd

³Dept. of Biomedical Laboratory Science, Soonchunhyang University

Correspondence to：류덕선(Deok-Seon Ryu) (31538) 충남 아산시 신창면 순천향로 22 임상병리학과(의료과학관 1114호) Tel：+82-41-530-3093, Fax：+82-41-530-3085, E-mail : duckssun@naver.com

Received July 18, 2017 Revised August 9, 2017 Accepted August 9, 2017

Abstract

Objectives

The purpose of this study was to investigate the effects of Angelica gigas Nakai extract(AGNE) and Bacillus polyfermenticus KJS-2 (BP2) on atopic dermatitis (AD) induced by 2, 4-dinitrochlorobenzene (DNCB) in mice.

Methods

In the experiment, we divided mice into four groups: a control group, a DNCB group, an AGNE group, and an AGNE+BP2 group. Then we examined the changes in scratching frequency, clinical aspects on dorsum skin, immunoglobulin (IgE), cytokines (TNF-α, IL-6) and expression of COX-2.

Resutls

From the experiment, the scratching frequency was significantly dropped in AGNE group and AGNE+BP2 group. Clinical observations of dorsum skin, there were a severe keratotic lesion and drop of dead skin cell in DNCB group, but symptoms of AD were decreased 39.6% in AGNE group and 49.6% in AGNE+BP2 group during 3 weeks. IgE, TNF-α and IL-6 were decreased significantly in both AGNE and AGNE+BP2 group. Expression of COX-2 was also decreased significantly in both groups.

Conclusions

In conclusion, these data suggest that AGNE can decrease symptoms of AD and BP2 makes AGNE more effective. So AGNE can be useful herbal therapy for AD.

Keywords: Angelica gigas Nakai extract (AGNE), Bacillus polyfermenticus KJS-2 (BP2), 2,4-dinitrochlorobenzene (DNCB), Atopic dermatitis (AD)

Fig. 1

Representative high-performance liquid chromatography profile (A) Decursin and decursinol angelate of standard materials (B) AGNE sample.

Fig. 2

The effect of AGNE and AGNE+BP2 on scratching behavior induced by compound 48/80 in ICR mice. AGNE(20 mg/kg), AGNE(20 mg/kg)+BP2(10⁷ CFU) administered orally on the scratching behavior induced by compound 48/80 (50 μg/mouse, s.c.) in mice. AGNE and AGNE+BP2 significantly inhibited the compound 48/80-induced scratching behavior than DNCB group. Each datum represents the means ± S.E.M. of three independent experiments. (*p<0.05 vs. normal group, #p<0.05 vs. compound 48/80-treated group).

Fig. 3

The effect of AGNE and AGNE+BP2 on the DNCB-induced atopic dermatitis. The BALB/c mice (n=6) were sensitized with 150 μL of 0.5% DNCB in acetone–olive oil(3:1) or vehicle applied to the dorsal skin twice each week for a total period of 3 weeks. After 2 weeks, AGNE(20 mg/kg) and AGNE(20 mg/kg)+BP2(10⁷ CFU) was orally administered 1 week prior to the end of the experiment. (A) Comparison of skin manifestation in all groups for 3 weeks (B) SCORAD index of all groups in 0week and 2 weeks. (*p<0.05 vs. normal group, #p<0.05 vs. compound 48/80-treated group).

Fig. 4

The effect of AGNE and AGNE+BP2 on the DNCB-induced IgE level in serum from mice. Blood samples were collected and then the levels of serum IgE in the indicated groups were measured using ELISA method. AGNE and AGNE+BP2 significantly inhibited the DNCB-induced IgE level in serum. Each datum represents the means ±± S.E.M. of three independent experiments(^*p<0.05 vs. normal group, ^#p<0.05 vs. DNCB-treated group, ^$p<0.05 vs. DNCB+AGNE 20 mg/kg-treated group).

Fig. 5

The effect of AGNE and AGNE+BP2 on the DNCB-induced (A) TNF-α and (B) IL-6 levels in serum from mice. Blood samples were collected and then the levels of serum TNF-α and IL-6 in the indicated groups were measured using ELISA method. AGNE+BP2 significantly inhibited the DNCB-induced TNF-α and IL-6 level in serum. Each datum represents the means ± S.E.M. of three independent experiments (^*p<0.05 vs. normal group, ^#p<0.05 vs. DNCB-treated group).

Fig. 6

The effect of AGNE and AGNE+BP2 on the DNCB-induced COX-2 levels in tissue from mice. Tissue samples were collected and then the levels of tissue COX-2 protein in the indicated groups were measured using Western blotting method. AGNE+BP2 significantly inhibited the DNCB-induced COX-2 level in tissue. Each datum represents the means ± S.E.M. of three independent experiments (^*p<0.05 vs. normal group, ^#p<0.05 vs. DNCB-treated group).

Table 1

Intensity Criteria of SCORAD Index

Symptoms	Score
Erythema	0 : None 1 : Mild 2 : Moderate 3 : Severe (Maximum : 18)
Edema/Papulation
Oozing/Crusting
Excoriation
Lichenification
Dryness

Table 2

Contents of Decursin, Decursinol Angelate, and Polysaccharides from AGNE

%	Decursin	Decursinol angelate	Polysaccharides
AGNE	34.11	14.98	46.72

Table 3

Contents of a Simple Sugar and Amino Sugar from AGNE

μg/g	Glucose	Galactose	Mannose	Fructose	Glucosamine	Galatosamine
AGNE	2475.0	53.3	53.3	2.7	11.3	8.8

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