AbstractObjectivesThe purpose of this study is to investigate changes of the marker compounds and anti-inflammatory effect of Gamisoyo-san decoction (GMSYS) depending on storage temperature and periods.
MethodsGMSYS was stored at room temperature or refrigeration for 12 months. According to storage temperature and periods, pH and sugar content of GMSYS were measured. To determine the marker compounds of GMSYS, high-performance liquid chromatography analysis was performed. To estimate the anti-inflammatory effect of GMSYS, LPS-induced pro-inflammatory mediators and cytokines were measured in RAW 264.7 cells.
ResultsThere was no change in pH and sugar content depending on storage temperature and periods of GMSYS. The contents of gallic acid and mangiferin in both of room temperature and refrigerated decoctions reduced with increasing storage periods. Chlorogenic acid was time-dependently decreased in case of stored at room temperature. GMSYS significantly inhibited the LPS-induced production of nitric oxide, prostaglandin E2 (PGE2) and IL-6 in RAW 264.7 cells. These effects equally maintained up to 3 months at both of room temperature and refrigeration. Since 4 months, the inhibitory effect of GMSYS on LPS-induced PGE2 production was time-dependently reduced, and the decrease in PGE2 inhibitory effect of decoction stored at refrigeration was lower than that of stored at room temperature.
참고문헌1. The co-textbook publishing committee of Korean Medicine College. Formula Science. Seoul. Younglimsa;(2009.
2. Seo, CS, Shin, HK, Kim, JH, & Shin, KS. Changes of Principal Components and Microbial Population in Pyungwi-san Decoction According to the Preservation Temperature and Period. J Korean Oriental Med, (2011). 32(5), 41-9.
3. Ha, H, Shin, IS, Lim, HS, Jeon, WY, Kim, JH, Seo, CS, & Shin, HK. Changes in Anti-inflammatory Effect of Pyungwi-san Decoction According to the Preservation Temperature and Period. Formula Science, (2012). 20(2), 29-35.
4. Seo, CS, Kim, JH, Lim, SH, & Shin, HK. Establishment of Shelf-Life of Ssanghwa-tang by Long-term Storage Test. Kor J Pharmacogn, (2012). 43(3), 257-64.
5. Seo, CS, Kim, JH, Kim, SS, Lim, SH, & Shin, HK. Evaluation of Shelf-life of Bojungikgi-tang by Long-term Storage Test. Kor J Pharmacogn, (2013). 44(2), 200-8.
6. Jin, SE, Kin, OS, Shin, HK, & Jeong, SJ. Comparative Study on Biological Activities of Gwakhyangjeonggi-san Decoction According to the Preservation Periods. J Korean Med, (2014). 35(3), 60-9.
7. Jin, SE, Kim, OS, Seo, CS, Shin, HK, & Jeong, SJ. Comparative study on stability and efficacy of Banhasasim-tang decoction depending on the preservation temperature and periods. J Korean Med, (2016). 37(1), 21-33.
8. Yoo, SR, Ha, H, Lee, NR, Shin, HK, & Seo, CS. A Study on Change of Marker Compounds and Biological Activity in Chungsimyeonja-eum Decoction Depending on A Storage Temperature and Periods. Kor J Herbol, (2017). 32(4), 25-32.
9. Park, IH, Kim, YH, Choi, SH, Yu, SN, Kim, SH, Ahn, SC, Cho, SI, & Lee, I. Effect of Preservation Conditions on the Stability of Samul-tang Decoctions. J Life Sci, (2015). 25(10), 1124-31.
10. Do, HJ, Shim, YS, Lee, JH, Ahn, YJ, Ha, IH, & Lee, YJ, et al. Stability of Danggwisu-san (Dangguixu-san) Water Extract, a Herbal Medicine, Under Various Storage Conditions. Journal of Oriental Rehabilitation Medicine, (2016). 26(4), 1-8.
11. Jin SM. Taepyunghyeminhwajekukbang. Inminweeseng Publications;(1985). p. 308
12. Sim, TK, Jung, IC, & Lee, SR. The Effect of Gami soyo-san(Jiaweixiaoyaosan) on Serotonin Metabolism. Journal of Oriental Neuropsychiatry, (2011). 22(1), 37-51.
13. Lee, SL, Lee, IS, & Soh, KS. Effects of Gamisoyosan(Jiaweixiaoyaosan 加味逍遙散) on Type II Collagen-Induced Arthritis. J Oriental Rehab Med, (2001). 12(4), 167-76.
14. Jin, SE, Kim, OS, Yoo, SR, Seo, CS, Kim, Y, & Shin, HK, et al. Anti-inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo-san in RAW 264.7 macrophages. BMC Complement Altern Med, (2016). 16, 219. 10.1186/s12906-016-1197-7
![]() ![]() 15. Choi, HM, Kim, SJ, Kim, IS, Lee, JB, Kim, JB, & Moon, SO, et al. Evaluation on Anti-oxidant Activity and Anti-inflammatory Effects for the New Formulation of Gamisoyosan. Kor J Herbol, (2016). 31(6), 1-9.
16. Willoughby, DA. Human arthritis applied to animal models. Towards a better therapy. Ann Rheum Dis, (1975). 34, 471-8.
![]() ![]() 17. Lee, ES, Ju, HK, Moon, TC, Lee, E, Jahng, Y, & Lee, SH, et al. Inhibition of nitric oxide and tumor necrosis factor α (TNF-α) production by propenone compound through blockade of nuclear factor (NF)-κB activation in cultured murine macrophages. Biol Pharm Bull, (2004). 27, 617-20.
![]() 18. Wink, DA. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med, (1998). 25(4–5), 434-56.
![]() 19. Ryu, JH, Ahn, H, Kim, JY, & Kim, YK. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages. Phytother Res, (2003). 17(5), 485-9.
![]() 20. Funk, CD, & Fitzgerald, GA. Human platelet, erythroleukemia cell prostaglandin G, H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J, (1991). 5, 2304-12.
21. Bishop-Bailey, D, Calatayud, S, Warner, TD, Hla, T, & Michell, A. Prostaglandins and the regulation of tumor growth. J Environ Pathol Tox Oncol, (2002). 21, 93-101.
23. Mann, JR, Backlund, MG, & DuBois, RN. Mechanism of disease: Inflammatory mediators and cancer prevention. Nat Clin Pract Oncol, (2005). 2, 202-10.
24. Guzik, TJ, Korbut, R, & Adamek-Guzik, T. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Phamacol, (2003). 43, 469-87.
25. Chiu, CS, Deng, JS, Chang, HY, Chen, YC, Lee, MM, & Hou, WC, et al. Antioxidant and anti-inflammatory properties of Taiwanese yam (Dioscorea japonica Thunb. var. pseudojaponica (Hayata) Yamam.) and its reference compounds. Food Chem, (2013). 141, 1087-96.
![]() 26. Hwang, SJ, Kim, YW, Park, Y, Lee, HJ, & Kim, KW. Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells. Inflamm Res, (2014). 63, 81-90.
![]() 27. Garrido, G, Delgado, R, Lemus, Y, Rodríguez, J, García, D, & Núñez-Sellés, AJ. Protection against septic shock and suppression of tumor necrosis factor alpha and nitric oxide production on macrophages and microglia by a standard aqueous extract of Mangifera indica L. (VIMANG). Role of mangiferin isolated from the extract. Pharmacol Res, (2004). 50, 165-72.
![]() 28. Shi, Q, Cao, J, Fang, L, Zhao, H, Liu, Z, & Ran, J, et al. Geniposide suppresses LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating NF-kB, MAPK and AP-1 signaling pathways in macrophages. Int Immunopharmacol, (2014). 20, 298-306.
![]() 29. Wang, QS, Gao, T, Cui, YL, Gao, LN, & Jiang, HL. Comparative studies of paeoniflorin and albiflorin from Paeonia lactiflora on anti-inflammatory activities. Pharm Biol, (2014). 52(9), 1189-95.
![]() 30. Jeong, HW, Hsu, KC, Lee, JW, Ham, M, Huh, JY, & Shin, HJ, et al. Berberine suppresses proinflammatory responses through AMPK activation in macrophages. Am J Physiol Endocrinol Metab, (2009). 296, E955-64.
![]() 31. Guan, Y, Li, FF, Hong, L, Yan, XF, Tan, GL, & He, JS, et al. Protective effects of liquiritin apioside on cigarette smoke-induced lung epithelial cell injury. Fundam Clin Pharmacol, (2012). 26(4), 473-83.
![]() 32. Rim, HK, Cho, W, Sung, SH, & Lee, KT. Nodakenin suppresses lipopolysaccharide-induced inflammatory responses in macrophage cells by inhibiting tumor necrosis factor receptor-associated factor 6 and nuclear factor-kB pathways and protects mice from lethal endotoxin shock. J Pharmacol Exp Ther, (2012). 342, 654-64.
33. Fu, Y, Zhou, E, Wei, Z, Liang, D, Wang, W, & Wang, T, et al. Glycyrrhizin inhibits the inflammatory response in mouse mammary epithelial cells and a mouse mastitis model. FEBS J, (2014). 281, 2543-57.
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