Protective effects of Puriton<sup>®</sup> on acute pancreatitis

Dong-Uk Kim; Bitna Kweon; Kwang-Ho Kim; Gi-Sang Bae; Kyung Song; Kim, Dong-Uk; Kweon, Bitna; Kim, Kwang-Ho; Bae, Gi-Sang; Song, Kyung

doi:10.13048/jkm.24045

JKM > Volume 45(3); 2024 > Article

Kim, Kweon, Kim, Bae, and Song: Protective effects of Puriton® on acute pancreatitis

Original Article

The Journal of Korean Medicine 2024; 45(3): 143-153.

Published online: September 1, 2024

DOI: https://doi.org/10.13048/jkm.24045

Protective effects of Puriton^® on acute pancreatitis

Dong-Uk Kim¹^{, 2}^{, §}

, Bitna Kweon^§

, Kwang-Ho Kim⁷

, Gi-Sang Bae¹^{, 2}^{, 3}^{, *}

, Kyung Song⁴^{, 5}^{, 6}^{, *}

¹Department of Pharmacology, College of Korean Medicine, Wonkwang University

²Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University

³Research center of Traditional Korean medicine, Wonkwang University

⁴Department of Pharmacy, College of Pharmacy, Wonkwang University

⁵Institute of Pharmaceutical Research and Development, Wonkwang University

⁶Quantum NS, Co., Ltd., Iksan, Jeollabuk-do, 54538, Republic of Korea

⁷Kadesh, Inc., Garden Grove, CA 92841, USA

Correspondence to: Gi-Sang Bae, Department of Pharmacology, College of Korean Medicine, Wonkwang University, 460 Iksandae-ro, Iksan, 54538 Jeonbuk, South Korea, Tel: +82-63-850-6842, E-mail: baegs888@wku.ac.kr

Correspondence to: Kyung Song, College of Pharmacy, and Institute of Pharmaceutical Research and Development, Wonkwang University, 460 Iksandae-ro, Iksan, 54538, Republic of Korea, Tel: +82-63-850-6817, Fax: +82-63-853-6821, E-mail: kyungsong@wku.ac.kr

§ These authors equally contributed.

Received July 23, 2024 Revised August 7, 2024 Accepted August 19, 2024

Abstract

Objectives

Puriton^® is the electrolyte enriched water consisting of 31 essential minerals from biotite, kaolinite, montmorillonite, serpentine, and clinochlore, and vermiculite. It has been reported to be bactericidal and virucidal. However, the protective effect of Puriton^® against acute pancreatitis (AP) has not yet been studied. Therefore, we aimed to evaluate the protective effect of Puriton^® against cerulein-induced AP.

Methods

AP was induced by intraperitoneal injections of cerulein (50 μg/kg) hourly for 6 times. Puriton^® (100, 300, 500, or 700 μL) was administered orally 1 hour before the first cerulein injection. Mice were sacrificed 6 hours after the last cerulein injection. Pancreas, lung, and serum samples were quickly collected for further analysis.

Results

Administration of Puriton^® did not reduce the ratio of pancreas weight to body weight. However, the increased serum amylase and lipase were decreased in the Puriton^® administration group, and histological damage to pancreas and lung tissue was suppressed in the Puriton^® 100 and 300 μL administration groups, but not in the Puriton^® 500 and 700 μL administration groups. Additionally, among pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, the mRNA level of only IL-6 was decreased by Puriton^® administration.

Conclusion

In summary, we showed that the administration of Puriton^® improved the severity of cerulein-induced AP, suggesting the possibility of being an effective drug for AP.

Keywords: Puriton®, Acute Pancreatitis (AP), Cytokine

Fig. 1

Effect of Puriton^® on pancreas weight/body weight (P.W./B.W.) ratio during cerulein-induced acute pancreatitis (AP). Data show the mean±S.E. for 9 mice for each group. Results are representative of three experiments. ^*p<0.05 vs control group, ^†p<0.05 vs cerulein treatment group.

Fig. 2

Effect of Puriton^® on the serum digestive enzymes during cerulein-induced AP. Serum (A) amylase and (B) lipase were measured as described in materials and methods. Data show the mean±S.E. for 9 mice for each group. Results are representative of three experiments. ^*p<0.05 vs control group, ^†p<0.05 vs cerulein treatment group.

Fig. 3

Effect of Puriton^® on morphological change of pancreas during cerulein-induced AP. (A) Representative hematoxylin & eosin (H&E)-stained sections of the pancreas. Histological scores for (B) edema and (C) inflammation. Data show the mean±S.E. for 9 mice for each group. Results are representative of three experiments. ^*p<0.05 vs control group, ^†p<0.05 vs cerulein treatment group.

Fig. 4

Effect of Puriton^® on morphological change of lung during cerulein-induced AP. (A) Representative hematoxylin & eosin (H&E)-stained sections of the pancreas. Histological scores for (B) alveolar membrane thickening and (C) inflammation. Data show the mean±S.E. for 9 mice for each group. Results are representative of three experiments. ^*p<0.05 vs control group, ^†p<0.05 vs cerulein treatment group.

Fig. 5

Effect of Puriton^® on the mRNA level of pro-inflammatory cytokines during cerulein-induced AP. The pro-inflammatory cytokines were detected by real time RT-PCR as described in materials and methods. Data show the mean±S.E. for 9 mice for each group. Results are representative of three experiments. ^*p<0.05 vs control group, ^†p<0.05 vs cerulein treatment group.

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