The Effect of Jeokbaekhaogwanjung-tang Herbal Acupuncture Solution on the Immune Activity of Spleen Cells of Aged Rats

Article information

J Korean Med. 2016;37(1):90-100

Publication date (electronic) : 2016 March 31

doi : https://doi.org/10.13048/jkm.16009

Hyun-Suk Lee, Kang-Min Choi, Yun-Kyoung Yim

Department of Meridians & Acupoints, College of korean Medicine, Daejeon University

Correspondence to: 임윤경, 대전 동구 대학로 62 대전대학교 12407호, Tel: +82-42-280-2610, Fax: +82-42-280-2610, E-mail: docwindy@dju.kr

Received 2016 February 16; Revised 2016 March 25; Accepted 2016 March 28.

Abstract

Objectives:

The purpose of this study was to investigate the effect of Jeokbaekhaogwanjung-tang (JGT) distillate on the immune activity of spleen cells of aged rats.

Methods:

Spleen cells of 10w, 52w 72w old SD rats were cultured and treated with JGT distillate and the levels of IL-2, IL-4, IL-10 and IFN-γ were measured.

Results:

The levels of IL-2 and IFN-γ in the spleen cells of 52w old rats were significantly decreased by JGT treatment.
The level of IFN-γ in the spleen cells of 72w old rats were significantly decreased by JGT treatment.

Conclusion:

These results suggest that Jeokbaekhaogwanjung-tang distillate has an immune regulative effect by way of suppressing the change of immune activity caused by aging in rats.

Keywords: Jeokbaekhaogwanjung-tang; immune activity; spleen cells; cytokine; aged rats

Fig. 1.

Effect of Aging on the levels of various immune cytokines in the spleen cell culture.

The levels of various immune cytokines in rat’s spleen cell culture were analysed by ELISA. Values represent mean ± SD (n=5) NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, **: p<0.01 compared to 10w group. †: p<0.05 compared to 52w group.

Fig. 2.

Effect of JGT distillate on IL-2 level in spleen cell culture of aged rat

The level of IL-2 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, **: p<0.01 compared to NT group.

†: p<0.05 compared to ConA group. ‡: p<0.05 compared to Vit.C group.

Fig. 3.

Effect of JGT distillate on IL-4 level in spleen cell culture of aged rat

The level of IL-4 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, *: p<0.05 compared to NT group.

††† : p<0.001, †† : p<0.01 compared to ConA group. ‡: p<0.01 compared to Vit.C group.

Fig. 4.

Effect of JGT distillate on IL-10 level in spleen cell culture of aged rat

The level of IL-10 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, **: p<0.01 compared to NT group.

††† : p<0.001 ††: p<0.01 compared to ConA group. ‡‡: p<0.01, ‡: p<0.05 compared to Vit.C group.

Fig. 5.

Effect of JGT distillate on IFN-γ level in spleen cell culture of aged rat

The level of IFN-γ in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours. ^*** : p<0.001 compared to NT group.

^†††: p<0.001 ^†: p<0.05 compared to ConA group. ^‡‡: p<0.01 compared to Vit.C group.

Table 1.

The Compositions of Jeokbaekhaogwanjung-tang(JGT)

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Article information Continued

Fig. 1.

Effect of Aging on the levels of various immune cytokines in the spleen cell culture.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, **: p<0.01 compared to 10w group. †: p<0.05 compared to 52w group.

Fig. 2.

Effect of JGT distillate on IL-2 level in spleen cell culture of aged rat

The level of IL-2 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, **: p<0.01 compared to NT group.

†: p<0.05 compared to ConA group. ‡: p<0.05 compared to Vit.C group.

Fig. 3.

Effect of JGT distillate on IL-4 level in spleen cell culture of aged rat

The level of IL-4 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, *: p<0.05 compared to NT group.

††† : p<0.001, †† : p<0.01 compared to ConA group. ‡: p<0.01 compared to Vit.C group.

Fig. 4.

Effect of JGT distillate on IL-10 level in spleen cell culture of aged rat

The level of IL-10 in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours.

***: p<0.001, **: p<0.01 compared to NT group.

††† : p<0.001 ††: p<0.01 compared to ConA group. ‡‡: p<0.01, ‡: p<0.05 compared to Vit.C group.

Fig. 5.

Effect of JGT distillate on IFN-γ level in spleen cell culture of aged rat

The level of IFN-γ in rat’s spleen cell culture was analysed by ELISA. Values represent mean ± SD (n=5)

NT : Cells with no treatment. ConA : Cells treated with ConA for 24 hours.

Vit.C : Cells treated with ConA and Vitamin C for 24 hours. JGT : Cells treaed with ConA and JGT distillate for 24 hours. ^*** : p<0.001 compared to NT group.

^†††: p<0.001 ^†: p<0.05 compared to ConA group. ^‡‡: p<0.01 compared to Vit.C group.

Table 1.

The Compositions of Jeokbaekhaogwanjung-tang(JGT)

Herbs	Pharmacognostic name	Dose(g)
白何首烏	Cynanchi Wilfordii Radix	4
赤何首烏	Polygoni Multiflori Radix	4
良薑	Alpiniae Officinari Rhizoma	4
乾薑	Zingiberis Rhizoma	4
陳皮	Citri Unshii Pericarpium	4
靑皮	Citrii Unshiu Immaturi Pericarpium	4
香附子	Cyperi Rhizoma	4
益智仁	Aipiniae Fructus	4
棗二	Zizyphi Fructus	7

Total		39