A Comparative Study of Sepiae Os, Arcae Concha, Ostreae Concha and Esomeprazole in a Mouse Model of Reflux Esophagitis

Article information

J Korean Med. 2018;39(2):92-105

Publication date (electronic) : 2018 June 30

doi : https://doi.org/10.13048/jkm.18018

Dept. of Internal Medicine, College of Korean Medicine, Sangji University

Correspondence to: 백태현(Tae-Hyun Baek), 강원도 원주시 우산동 283번지, 상지대학교 부속한방병원 내과학교실, Tel: +82-33-741-9207, E-mail: white@sangji.ac.kr

Received 2018 May 31; Revised 2018 June 20; Accepted 2018 June 20.

Abstract

Objectives

This aim of this study is to compare the reflux esophagitis improvement effects of Sepiae Os, Arcae Concha, Ostreae Concha, and Proton Pump Inhibitor(esomeprazole) through rat experiments.

Methods

NO production inhibitory effect was measured by NO production amount and iNOS mRNA expression level in cell lines. iNOS, TNF-α and p-IκB, and serotonin were compared using immunohistochemistry at the rat reflux esophagitis. Reflux esophagitis connection external form, lower esophageal sphincter, and gap were observed and an esophageal inflammatory indicator, IL-6 activity was also evaluated by immunohistochemistry.

Results

NO production and iNOS mRNA expression was showed concentration dependent decrease in cell lines treated with Sepiae OS, Arcae Concha, and Ostreae Concha at the experiments of cell lines. In the suppression of iNOS and p-IκB at the rat reflux esophagitis, Sepiae Os treat group(SOT) and Ostreae Concha treat group(OCT) were more effective. In the increase of serotonin at the rat reflux esophagitis, ACT, MT and OCT were more effective. Damage of lower esophageal sphincter, and gap between esophageal keratin and mucosa were observed less at the SOT, ACT, OCT. In the suppression of IL-6 at the rat reflux esophagitis, SOT and OCT were more effective than GE and, SOT was more effective than MT significantly.

Conclusions

The anti-inflammatory effect was the best in the SOT and lower esophageal sphincter muscle contraction was the best in the ACT at the rat reflux esophagitis. Sepiae OS was more effective than esomeprazole in the suppression of iNOS, TNF-α, and IL-6.

Keywords: Sepiae Os; Arcae Concha; Ostreae Concha; esomeprazole; reflux esophagitis

Fig. 1

Experimental design and reflux esophagitis animal model

Abbreviation. HFD, high fat diet; SO, Sepiae Os; AC, Arcae Concha; OC, Ostreae Concha.

Fig. 2

Inhibition of NO production and iNOS mRNA expression by Sepiae Os (SO)

Fig. 3

Inhibition of NO production and iNOS mRNA expression by Arcae Concha (AC)

Fig. 4

Inhibition of NO production and iNOS mRNA expression by Ostrea Concha (OC)

Fig. 5

The iNOS positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis

Abbreviation. Ctrl, no reflux esophagitis elicited group; GE, reflux esophagitis elicited group; MT, medicine as esomeprazole treat group before reflux esophagitis elicitation; SOT, Sepiae Os (SO) treat group before reflux esophagitis elicitation; ACT, Arcae Concha (AC) treat group before reflux esophagitis elicitation; OCT, Ostreae Concha (OC) treat group before reflux esophagitis elicitation; Bar size, 200μm; arrow, iNOS positive reaction; LES, lower esophageal sphincter; ES, esophagus; LP, lamina propria.

Fig. 6

The TNF-α positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis

Fig. 7

The p-IκB positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis

Fig. 8

The Serotonin positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis

Fig. 9

The improvement effect of hemorrhagic erosion in GEJ by SO, AC, OC and Esomeprazole (a photo ×4; b photo bar size 200μm)

Fig. 10

The IL-6 positive reaction in gastroesophageal junction (GEJ) with reflux esophagitis

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