Effects of Red Ginseng-Ejung-tang Water Extract on Production in LPS-induced Mouse Macrophages

Park, Wansu; Park, Wansu

ORIGINAL ARTICLE

J Korean Med. 2012;33(4): 42-49.

Effects of Red Ginseng-Ejung-tang Water Extract on Production in LPS-induced Mouse Macrophages

Wansu Park

Dept. of Pathology, College of Korean Medicine, Gachon University

Corresponding Author: Wansu Park ,Tel: +82-31-750-8821, Fax: +82-31-750-8821, Email: hangl98@naver.com

Received: September 8, 2012; Accepted: December 7, 2012.

ABSTRACT

Objectives: The purpose of this study was to investigate effects of Red Ginseng-Ejung-tang Water Extract (ER) on cytokine production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS).
Methods: Levels of various cytokines such as interleukin (IL)-6, IL-10, IL-2, IL-12p70, vascular endothelial growth factor (VEGF), monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-2, keratinocyte-derived chemokine (KC), tumor necrosis factor (TNF)-alpha, granulocyte macrophage colony-stimulating factor (GM-CSF) were measured by high-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology.
Results: ER significantly decreased levels of IL-6, IL-10, IL-2, IL-12p70, VEGF, and MCP-1 for 24 hrs incubation at the concentrations of 25, 50, and 100 ??mL in LPS-induced RAW 264.7 cells (P < 0.05). But ER did not exert significant effects on production of MIP-2, KC, TNF-慣, and GM-CSF in LPS-induced RAW 264.7 cells.
Conclusions: These results suggest that ER has an anti-inflammatory property related with its inhibition of cytokine production in LPS-induced macrophages.

Keywords: Red ginseng | Ejung-tang | macrophage | inflammation | cytokine